Improved Method for the Preparation and Purification of Boar mα-Acrosin
نویسندگان
چکیده
منابع مشابه
Boar acrosin. I. Purification and preliminary characterization of a proteinase from boar sperm acrosomes.
Acrosin is a proteolytic enzyme used by sperm to digest a path through the zona pellucida of the ovum. In ejaculated sperm it is inactivated by a proteinase inhibitor from seminal plasma that also inhibits trypsin. This inhibitor is removed or inactivated during the residence in the female reproductive tract as a part of the capacitation process. The boar acrosin-inhibitor complex was partially...
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the current study aimed at investigating the relationship between motivation and test preparation strategies (tpss) used by iranian pre-university students in their preparation period for the university entrance exam (uee). due to the importance of uee in iran, this study also attempted to show its impact on these two important variables. to this end, 100 pre-university students in an iranian p...
Proacrosin conversion inhibitor. Purification and initial characterization of a boar sperm protein which prevents the conversion of proacrosin into acrosin.
A proacrosin conversion inhibitor present in boar spermatozoa has been purified and initially characterized. Purification methods included sequential acid extractions of washed spermatozoa at pH 4.0, pH 3.5, and pH 2.5 followed by successive gel filtrations of the pH 2.5 sperm extract supernatant over Sephadex G-75 and G-50. The resulting 8.8-fold purified materials were judged to be homogeneou...
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15 صفحه اولSimple and Efficient Method for the Preparation of 5-Bromoisatins
Isatin, N-methyl isatin and N-benzylisatin were smoothly monobrominated in the 5-position by treatment with pyridinium bromochromate (PBC) in acetic acid medium. In the similar conditions indigo underwent bromination and oxidation to give 5-bromoisatin in high yield.
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1981
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod25.1.197